No conjugate is needed for fluorescent blot detection. The smaller the size of the protein of interest, the higher the percentage of acrylamide/bis. one plane of separation) or two dimensional. Electrophoresis can be one dimensional (i.e. ![]() Chameleon 700 or Chameleon 800 protein ladders can be used for one-channel analysis. Download the complete western blot guide. Loading buffer: 30% (w/v) glycerol, 2% SDS, 62.5 mM Tris, pH 6.8, 50 mM DTT, 5 mM EDTA, 0.02% NaN 3, 0.01% bromophenol blue.įluorescent detection: prepare a 1:10 dilution of the WesternC Standard and load 5 µl per well. Ensure good size coverage with Chameleon ladders ranging from 8 kDa to 260 kDa Easily visualize gel migration and protein size Orient your gel or membrane quickly Chameleon Pre-stained Protein Ladders are optimized for use with Bis-Tris and Tris-Glycine gels. Packaging Options Number of Applications (Volume) Choose from a variety of protein ladders (molecular weight markers) for protein electrophoresis and western blotting applications. Thermo Scientific PageRuler Unstained High Range Protein Ladder is a mixture of eight (8) unstained proteins (60 to 250kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and Western blotting. The protein ladder is supplied in a ready-to-use format for direct loading onto gels no need to heat, reduce, or add sample buffer prior to use. Fluorescent blot detection of prestained bands, with blue bands visualized in the red wavelength range and pink bands in the green wavelength range Thermo Scientific PageRuler Unstained Broad Range Protein Ladder is a mixture of 11 proteins (5 to 250 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and western blotting.Artifacts from overloading the protein marker or ladder: Load less of the molecular weight marker onto the gel. Place this handy selection guide magnet on your refrigerator or lab bench to eliminate the guesswork and make protein standard selection simple. Change to membrane with smaller pore size. In this section, you can find solutions to issues related to protein band appearance. Molecular weight sizing of proteins after blot development Solve your western blot problems with this western blot troubleshooting guide, covering high background. The Western Blot Doctor is a self-help guide that enables you to troubleshoot your western blotting problems. ![]()
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